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BSE(BOVINE SPONGIFORM ENCERPHALOPATHY)-SPECIFIC CELL MODEL USING siRNA
BSE(BOVINE SPONGIFORM ENCERPHALOPATHY)-SPECIFIC CELL MODEL USING siRNA
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机译:使用siRNA的BSE(牛海绵状脑病)-特定细胞模型
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摘要
PURPOSE: A BSE(Bovine Spongiform Encephalopathy)-specific cell model is provided to use as a basic device for pathogenic mechanism or biomolecular research of diseases.;CONSTITUTION: A method for producing BSE(Bovine Spongiform Encephalopathy)-specific cell model comprises: a step of cloning bovine prion gene(boPrnp ORF, sequence number 4) into an expression vector of eukaryotic cell; a step of transplanting the vector into nerve cell line through transfection carrier; and a step of inducing siRNA to mouse nerve cell line to suppress the expression of mouse prion protein. The mouse nerve cell line is N2a(mouse neuroblastoma).;COPYRIGHT KIPO 2010
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