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KIT AND METHOD FOR DIAGNOSING BOVINE PREGNANCY USING BOVINE FECES

机译:使用牛粪便诊断牛妊娠的试剂盒和方法

摘要

PURPOSE: A kit and a method for diagnosing bovine pregnancy are provided to test bovine pregnancy without giving stress to the bovine.;CONSTITUTION: A kit for diagnosing bovine pregnancy comprises: a strip member(10) which is porous thin film with a specific length; a test line(11) in which progesterone-BSA conjugate or progesterone-KLH conjugate on the surface of the strip member and which react with conjugate liquid containing anti-progesterone-colloid gold particle conjugate and bovine feces extract liquid; a control line(13) which is formed by coating anti-mouse IgG on the surface of the strip member and reacts with the conjugate liquid to compare the color degree of the test line; a absorb paper(40) which is integrally laminated on the surface of the strip member and absorbs the conjugate liquid from one end to the other end of the strip member; a sample pad(20) for transferring bovine feces extract to one end of strip member; and a gold conjugate pad(30) which is able to produce a conjugate liquid moving from one end to the other end of the strip member by binding the anti-progesterone-colloid gold particle conjugate with bovine feces extract liquid. The strip member is nitrocellulose membrane.;COPYRIGHT KIPO 2010
机译:目的:提供一种用于诊断牛妊娠的试剂盒和方法,以在不给牛带来压力的情况下测试牛的妊娠。组成:一种用于诊断牛妊娠的试剂盒,包括:条状构件(10),其为具有特定长度的多孔薄膜;试验线(11),在条状部件的表面上,孕酮-BSA结合物或孕酮-KLH结合物与含有抗孕酮-胶体金颗粒结合物的结合液和牛粪提取液反应。对照线(13),其通过在条带构件的表面上涂覆抗小鼠IgG而形成,并与结合液反应以比较测试线的色度;吸收纸(40),该吸收纸(40)被整体地层压在带状构件的表面上,并且从带状构件的一端到另一端吸收结合液。用于将牛粪提取物转移到条状构件的一端的样品垫(20);金共轭垫(30),其通过将抗孕酮-胶体金颗粒共轭物与牛粪便提取液结合,能够产生从条状构件的一端移动到另一端的共轭液。带状构件是硝酸纤维素膜。; COPYRIGHT KIPO 2010

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