METHOD FOR INDUCING AND PROLIFERATING SOMATIC EMBRYOGENIC CALLI INDUCED FROM ROOTS OF ROSE

摘要

PURPOSE: A method for inducing and proliferating somatic embryogenic callus is provided to develop a novel product of Rosa hybrida L. having effective characteristics. CONSTITUTION: A method for inducing somatic embryogenic callus from Rosa hybrida L. root comprises: a step of culturing Rosa hybrida L. in MS solid medium(pH 5.8), proliferating, and rooting; a step of culturing the fragment of the redifferentiated root in SH medium containing 5-8 mg/L of 2,4-dichlorophenoxyacetic acid for 2-8 weeks to induce callus; and a step of culturing the callus in SH medium to develop embryo for second callus induction.