Repetitive somatic embryogenesis induced cytological and proteomic changes in embryogenic lines of Pseudotsuga menziesii Mirb.

摘要

BackgroundTo explore poorly understood differences between primary and subsequent somatic embryogenic lines of plants, we induced secondary (2^ry) and tertiary (3^ry) lines from cotyledonary somatic embryos (SEs) of two Douglas-fir genotypes: SD4 and TD17. The 2^ry lines exhibited significantly higher embryogenic potential (SE yields) than the 1^ry lines initiated from zygotic embryos (SD4, 2155 vs 477; TD17, 240 vs 29 g^− 1 f.w.). Moreover, we observed similar differences in yield between 2^ry and 3^ry lines of SD4 (2400 vs 3921 g^− 1 f.w.). To elucidate reasons for differences in embryogenic potential induced by repetitive somatic embryogenesis we then compared 2^ry vs 1^ry and 2^ry vs 3^ry lines at histo-cytological (using LC-MS/MS) and proteomic levels.