METHOD FOR PRODUCING L-RIBOSE THROUGH REACTION OF RECOMBINANT MANNOSE-6-PHOSPHATE ISOMERASE AND L-RIBULOSE

摘要

PURPOSE: A method for producing L-ribose which is a raw material of a medicinal product using Bacillus subtilis-drived recombinant mannose-6-phosphate isomerase.;CONSTITUTION: A method for producing L-ribose comprises: a step of isolating mannose-6-phosphate isomerase gene and inserting to an expression vector; a step of transforming the expression vector to E.coli and culturing; a step of isolating mannose-6-phosphate isomerase from the E.coli and purifying; a step of reacting mannose-6-phosphate isomerase and L-ribulose as a substrate.;COPYRIGHT KIPO 2010