A method for preparing multipotent neural stem cells from women labia minora skin cells by reprogramming method, and use of reprogrammed neural stem cells as a cell therapy product

摘要

The present invention provides a variety of nerve cells through the process of re-programming (reprogramming) of adult cells derived from female labia minora Method for manufacturing a neural stem cell capable of differentiating to the (neural stme cells) and to the use as a cell therapeutic agent of the differentiated cell. Adult cells isolated from the women's labia minora according to the present invention, a low glucose (low glucose), while it was possible to mass proliferation of multipotent maintained when cultured in DMEM medium. However, the efficiency of culture for these multipotential bar which is significantly less for the adult cells to overcome the ITS + bFGF contained glucose (low glucose) DMEM / F12 3 ~ 7 days in serum-free medium of the following neural stem cells converted to N2 by the culture medium conditions necessary for growth as well as to promote the formation singyeonggu singyeonggu to promote proliferation of the multipotent neural stem cell in a quickly and easily could be obtained in large quantities. Neural stem cells are generated by the process of the mature cells and reprogrammed (reprogramming) can be used as a cellular therapeutic agent for a variety of diseases.