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Primer sets for qualitative analysis of insects resistant genetically modified Chinese cabbage

机译:定性分析抗虫转基因大白菜的引物组

摘要

A primer set for qualitative analysis of a genetically modified Chinese cabbage containing a primer which specifically amplifies endogenous gene and primer which specifically amplifies in a genetically modified Chinese cabbage having insect resistance is provided. A primer set for polymerase chain reaction(PCR) comprises a gene sequence of the sequence number 1 (Bra F-14) to 2 (Bra R-14) and specifically amplifies 121 bp in the endogenous gene of Chinese cabbage. The primer set for PCR contains gene sequence of the sequence number 3 (mBT120 F-1) to 4 (mBT120 R-1) and specifically amplifies 161 bp in the genetically modified Chinese cabbage having insect resistance. A qualitative analysis of the genetically modified Chinese cabbage having insect resistance is to verify with two primer sets through the PCR..
机译:提供了用于定性分析转基因大白菜的引物组,其包含特异性扩增内源基因的引物和特异性扩增具有抗虫性的转基因大白菜的引物。用于聚合酶链反应(PCR)的引物组包含序列号1(Bra F-14)至2(Bra R-14)的基因序列,并且特异性地扩增了大白菜的内源基因中的121 bp。用于PCR的引物组包含序列号3(mBT120 F-1)至4(mBT120 R-1)的基因序列,并且在具有昆虫抗性的转基因大白菜中特异性扩增161bp。对具有昆虫抗性的转基因大白菜的定性分析是通过PCR用两个引物对进行验证。

著录项

  • 公开/公告号KR100966127B1

    专利类型

  • 公开/公告日2010-06-25

    原文格式PDF

  • 申请/专利权人

    申请/专利号KR20080000249

  • 发明设计人 김해영;지상미;김재환;

    申请日2008-01-02

  • 分类号C12N15/10;C12N15/09;C12Q1/68;C12N15/11;

  • 国家 KR

  • 入库时间 2022-08-21 18:31:06

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