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PCR primer set related to heat stress tolerance in Chinese cabbage using HRM analysis

机译:应用HRM分析与大白菜耐热胁迫相关的PCR引物

摘要

The present invention relates to a PCR primer related to heat-resistance of Chinese cabbage using HRM analysis, a PCR kit for high resolution melting (HRM) analysis including the primer set for selecting high-temperature stress-resistant and sensitive Chinese cabbage, and a method for selecting high-temperature stress-resistant and sensitive Chinese cabbage, the method comprising: a step of mixing the primer set and total DNA samples isolated from Chinese cabbage for carrying out PCR, and performing HRM analysis by using amplified samples. The primer set according to the present invention is a PCR primer set for HRM analysis capable of identifying the existence of genes related to high-temperature stress-resistance and sensitivity of Chinese cabbage without analyzing phenotype and DNA sequence. As high-temperature stress-resistance and sensitivity related genes of 92 and 93 systems can be identified at low costs in a short time by using the primer set, developing and breeding Chinese cabbage varieties can be effectively improved.
机译:本发明涉及一种使用HRM分析的与大白菜耐热性有关的PCR引物,一种用于高分辨率熔解(HRM)分析的PCR试剂盒,其包括用于选择耐高温胁迫和敏感大白菜的引物组,以及一种选择抗高温胁迫敏感大白菜的方法,该方法包括:将引物组和从大白菜中分离的总DNA样品混合以进行PCR,并使用扩增的样品进行HRM分析的步骤。根据本发明的引物组是用于HRM分析的PCR引物组,其能够在不分析表型和DNA序列的情况下鉴定与大白菜的高温胁迫抗性和敏感性相关的基因的存在。通过使用引物组可以在短时间内低成本鉴定出92和93系统的高温抗逆性和敏感性相关基因,从而可以有效地改善大白菜品种的开发和育种。

著录项

  • 公开/公告号KR101492989B1

    专利类型

  • 公开/公告日2015-02-13

    原文格式PDF

  • 申请/专利权人

    申请/专利号KR20130030873

  • 发明设计人 정상민;알리 아스자드;

    申请日2013-03-22

  • 分类号C12N15/11;C12Q1/68;

  • 国家 KR

  • 入库时间 2022-08-21 14:58:39

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