Gene constructs comprising two or more reprogramming-inducing genes and method for preparing induced pluripotent stem cells using the same

摘要

The present invention provides a gene construct containing the gene coding for each of the 2 or more jong induced de-differentiation factor, the genes provides a gene construct linked via a DNA fragment (segment) encoding the peptide of SEQ ID NO: 1. Further, the present invention provides a multi-station differentiation-inducing gene (multiple reprogramming-inducing gene) expression plasmid containing the gene construct. In addition, the invention is the gene structure of the plasmid vector virus (viral vector plasmid) obtained by recombinant gene expression plasmid multiple de-differentiation inducing the receiving cell line (cell line permissive) in the packaging (packaging) to provide the resulting virus will be. The present invention also provides a method for producing the gene construct introduced into a somatic cell derived from human, and de-differentiation which comprises the step of culturing the somatic cell into which the construct introduced into iPS cells (induced pluripotent stem cells). ; De-differentiation, gene structure, re-programmed (reprogramming)