METHOD OF ENTEROCOCCI ISOLATION IN ANAEROBIC CONDITIONS FROM ACCUMULATION MEDIUM

摘要

FIELD: chemistry.;SUBSTANCE: without grinding, a sample of freshly collected pathological material is put into an accumulation medium - guanosine monophosphate broth with addition of 1% glucose and then cultured in a temperature-controlled cabinet at 37°C for 24 hours. Seeding is then carried out on a Petri dish with agar medium with addition of 5% erythrocytes and on enterococcus agar. The inoculations are kept in a temperature-controlled cabinet for 24-48 hours at 37°C. Samples which failed to grow in aerobic conditions are further inoculated on a Petri dish with agar medium with addition of 5% erythrocytes and then put into an anaerobic jar for 24 hours at 37°C. The inoculations are examined under a microscope and microorganisms are identified from the results using traditional methods.;EFFECT: higher isolation rate of enterococcus in anaerobic conditions at 31,2-36% and possibility of detecting presence of microorganisms in clinical material which do not grow in aerobic conditions.;1 tbl