METHOD OF THE DIRECTED DELIVERY OF DNA INTO TUMOR AND STEM CELLS

摘要

1. A method for targeted delivery of DNA in tumor and stem cells expressing the receptor CXCR4, by incorporating into the carrier molecule signal sequences, characterized in that the signal sequences for the receptor is made of the following sections: signal sequence to CXCR4 receptor at the cell surface; ! Linker portion of two molecules of ε-aminohexanoic acid linking a signal sequence to the DNA compacts; ! DNA-binding sequence consisting of eight amino acid residues of lysine - KKKKKKKK. ! 2. A method according to claim 1, characterized in that the signal sequence is a fragment of the sequence N-terminus of the protein SDF-1α- KPVSLSYR. ! 3. A method according to claim 2, characterized in that it further includes a signal sequence KPVSLSYR RFFESH. ! 4. A method according to claim 1, characterized in that as a signal portion using N-terminal sequence of the viral chemokine vMIP - LGASWHRPDK. ! 5. A method according to claim 1, characterized in that as the component that provides output from endosomes complexes consisting of carriers and genetic material using glycerol or chloroquine. ! 6. A method according to claim 1, characterized in that as the genetic material used for the carrier plasmid DNA. ! 7. A method according to claim 1, characterized in that as the genetic material is RNA oligonucleotide sequences and DNA. ! 8. A method according to claim 1, characterized in that the DNA-binding sequence consisting of carriers are peptides, polymer compound liposomes.