METHOD FOR DIRECTED DELIVERY OF DNA INTO TUMOR AND STEM CELLS

摘要

FIELD: medicine.;SUBSTANCE: invention may be used for directed delivery of genetic structures into stem and malignant tumor cells in order to correct gene defects and to prevent diseases. Method includes preparation of genetic structure carriers, by inclusion of carrier molecules, being a DNA-binding sequence of eight remains of lysine amino acid - KKKKKKKK, of signal sequences. Connection of signal sequence to DNA-binding sequence is carried out with the help of linker section of two molecules of ε-aminohexanoic acid. Afterwards complexes of DNA/carrier are formed. Then in vitro transfection is carried out.;EFFECT: increased efficiency of gene of interest delivery into tumor and stem cells.;3 cl, 4 dwg, 1 ex