BIOLUMINESCENT METHOD FOR DETECTION OF ANTIOXIDANT ACTIVITY OF HUMIC SUBSTANCES

摘要

FIELD: medicine.;SUBSTANCE: method includes preparation of working and control bioluminescent samples on the basis of ferments, induction period of both samples bioluminescence is registered, if ratio is T_hs/T° 1, then humic substances have antioxidant activity. In preparation of control sample, they use standard bioluminescent solution with addition of oxidant, and in preparation of working sample - standard bioluminescent solution with addition of oxidant, containing humic substances. Oxidant used is potassium ferricyanide in concentrations from 5·10^-6M to 3·10^-5M.;EFFECT: invention does not require complex equipment and expensive materials and reagents and is sensitive and express (10-30 min).;2 dwg, 1 ex