METHOD OF ISOLATION AND PURIFICATION OF DEOXYRIBONUCLEIC ACIDS

摘要

FIELD: medicine.;SUBSTANCE: sample is subjected to lysis in buffer on the basis of anionic detergent (SDS), lysate is subjected to purification and deoxyribonucleic acid is precipitated with ethanol or isopropanol. Lysate purification is performed by means of detonation nanodiamond, selectively sorbing mixtures of non-nuclear nature. Detonation nanodiamond is preliminarily burnt at temperature interval 400-700°C in vacuum 10^-2-10^-3 Torr. Obtained DNA solution contains both short and long fragments. Claimed method of isolation and purification of DNA allows to avoid application of such highly toxic compounds as phenol or chloroform. Contamination of obtained DNA with chemically active substances is prevented.;EFFECT: in case of accidental contact of detonation nanodiamond with final solution, polymerase chain reaction is not blocked, it is possible to separate non-soluble of detonation nanodiamond sediment from DNA solution.;3 dwg, 1 tbl, 4 ex