genetically modified dendritic cells, with adeno - associated virus transduziert, method for the production of these cells and their uses

摘要

The present invention is based upon the successful transduction of dendritic cells by an AAV vector containing a heterologous gene which is expressed in dendritic cells. The present invention is directed to a method for preparing genetically altered dendritic cells, wherein the method comprises: (a) obtaining dendritic cell precursors from a subject; and (b) transducing the dendritic cell precursors with at least one adeno-associated virus (AAV) vector comprising at least one heterologous gene to obtain genetically altered dendritic cells expressing said heterologous gene. After the transduction, the transduced precursors are incubated in a medium and under conditions whereby the dendritic cell precursors differentiate into dendritic cells that contain the heterologous gene. In a further embodiment, the present invention is directed to an adeno-associated virus (AAV) vector comprising at least a portion of the AAV genome and at least one heterologous gene selected from the group consisting of a gene encoding an antigen, a gene encoding a cytokine and combinations of one or more genes by genetically altered dendritic cells.