m - exchange in biological samples and the determination of structures of percentage of deuterium in biological samples

摘要

The present invention relates to a hydrogen isotopic analysis method, in particular, it is a determination method used for biological sample structure hydrogen deuterium substitution and deuterium percentage content. Said method includes the following steps: selecting a matrix, uniformly obtained bioloical sample and proper quantity of matrix and freeze-drying, fully combusting and oxidating the freeze-dried and mixed smaple by oxidant, oxidating hydrogen in mixed sample, into water, separating generated water and making it react with zinc to produce hydrogen gas, using gas isolope mass spectrometer to determine 2H/1H ratio value of hydrogen gas, then utilizing calculation formula to calculate percentage content of deuterium in biological sample. Said invention is simple in operation, and provides its application method and its advantages.