Recombinant mammalian cell for screening a substance preventing and treating aids and screening method using the same

摘要

The invention relates to recombinant mammalian cells for screening a substance for preventing and treating AIDS and a method of screening the substance using the cells. More particularly, disclosed are recombinant mammalian cells, transfected with an HIV NC expression cassette, which expresses an HIV NC protein, and with a reporter gene expression cassette, which expresses an HIV Psi (Psi) sequence and a reporter gene, as well as a screening method using the cells. The recombinant mammalian cells and the screening method provide a system capable of screening a substance, which inhibits the interaction between the HIV NC protein and the HIV Psi sequence, using eukaryotic cells. Accordingly, the disclosed invention provides novel means capable of screening a novel substance, which inhibits the interaction between the HIV NC protein involved in the packaging of HIV and the HIV Psi interaction. In addition, the present invention provides a novel method for screening a substance for preventing and treating AIDS.