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METHOD FOR EVALUATING SOIL HEALTH BY ACTIVITY MEASUREMENT OF PECTIN-DEGRADING ENZYME

机译:果胶降解酶活性测定评价土壤健康的方法

摘要

PROBLEM TO BE SOLVED: To provide a method for evaluating soil health which obtains the result in a short time while considering antagonism to other microorganisms in soil.;SOLUTION: The method for evaluating soil health by activity measurement of pectin-degrading enzyme includes (i) adding a pectin to a sample of soil of evaluated object, culturing the sample, and measuring a pectin-degrading enzyme activity after the culture, (ii) culturing the sample without adding a pectin to a sample of soil of evaluated object and measuring a pectin-degrading enzyme activity after the culture, (iii) obtaining a difference between the pectin degrading enzyme activity measured by (i) and the pectin-degrading enzyme activity measured by (ii), and making the difference as a pectin-degrading enzyme induction activity of the soil of evaluated object, and (iv) comparing the pectin-degrading enzyme induction activity of the soil of evaluated object with a previously calculated pectin-degrading enzyme induction activity of sound soil and determining the soil of evaluated object as unsound soil when the pectin-degrading enzyme induction activity of the soil of evaluated object is higher than the pectin-degrading enzyme induction activity of the sound soil by a fixed ratio.;COPYRIGHT: (C)2012,JPO&INPIT
机译:解决的问题:提供一种评估土壤健康的方法,该方法可在短时间内获得结果,同时考虑对土壤中其他微生物的拮抗作用;解决方案:通过果胶降解酶活性测量评估土壤健康的方法包括(i )在被评估对象的土壤样品中添加果胶,培养样品并在培养后测量果胶降解酶的活性,(ii)在不向评估对象的土壤样品中添加果胶的情况下培养样品并测量培养后的果胶降解酶活性,(iii)求出(i)测定的果胶降解酶活性与(ii)测定的果胶降解酶活性之差,作为果胶降解酶的诱导。被评估对象土壤的活性;(iv)将被评估对象土壤的果胶降解酶诱导活性与先前计算的果胶降解酶当被评估对象土壤的果胶降解酶诱导活性比固定土壤的果胶降解酶诱导活性高出固定比例时,将被评估对象的土壤活性确定为不健全土壤。 :(C)2012,JPO&INPIT

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