Its use and production of neutral metalloprotease in an environment free of serine protease

摘要

Preparing a Bacillus host cells with the endogenous serine alkaline protease enzyme, and endogenous extracellular neutral metalloprotease enzyme, and endogenous extracellular serine protease enzyme of the trace has been deleted, it is operatively associated with the promoter a nucleic acid encoding (NprE) enzyme heterologous neutral metalloprotease, the heterologous NprE enzyme, comprising the steps of preparing a host cell transformed by transforming a Bacillus host cell, the transformed host cells Method for producing a neutral metalloprotease extremely low and the step of producing a heterologous NprE enzyme is cultured under conditions suitable to be produced, the mixing of serine protease enzymes, and transformed and used in this way Bacillus host cells, and compositions comprising neutral metalloprotease obtained in this way. [Selection] Figure Figure 1