Methods and compositions for labeling oligonucleotides and analogs of synthesis on a solid support

摘要

(57) Abstract Possesses structural (i) (here as for S, to be solid backing, as for A, to be cutting possible linker, as for X part which possesses the binding site of three or more to be, as for L, to be sign, as for Y, to be nucleophilic reagent, and as for P₁, it is the cutting possible protective group with acid,) origonukureochido on the solid backing which and method and the composition which the sign do analog directly are offered. The solid backing which the sign is done to react with periodic style, on the solid backing 5 ' the labeling nucleotide is synthesized with the direction of empty 3 ', this labeling nucleotide has structural (ii). In addition as for the labeling nucleotide, it is synthesized origonukureochido and the (iii) coupling reagent on the solid backing which possesses the sign reagent (ii) nucleophilic functional group which possesses the functional group which consists of (i) carboxylic acid, sulfonic acid, phosphon acid or phosphoric acid, by reacting.