CombiClick™ Solid Support Based Combinatorial Click Chemistry for Efficient Oligonucleotide Conjugation: A New Versatile Method for Oligonucleotide Labeling

摘要

>RP-73The Copper (I) catalyzed Huisgen 1,3-dipolar cycloaddition between alkynes and azides discovered by the Sharpless group in 2002 (“click chemistry”), is a novel and very potent method for incorporation of molecules of interest (reporter molecules, lipophilic ligands, etc.) into oligonucleotides. Previously described post-synthetic methods permit the incorporation of only a single type of reporter moiety or multiple copies of the same reporter moiety into an oligonucleotide. We have developed a method for efficient attachment of a variety of dyes to the oligonucleotide backbone in nearly quantitative yields (95-98 %). This approach allows the synthesis of multiply labeled oligonucleotides in combinatorial fashion during oligonucleotide synthesis on solid support.