Propionibacterium = flue den Reiki over from a gene encoding an enzyme required for the biosynthesis of vitamin B12

摘要

Enzyme encoded by the genes and four novel is disclosed. Exist in the (Propionibacterium freudenreichii) Propionibacterium = flue Den Reiki over, the enzyme is required for the process of at least five of the biosynthetic pathway of vitamin B _12. Enzyme of the four are as follows: a acid Kobirinin, c- diamide synthase; B:: A enzyme has a (cobinamidephosphate guanylyltransferase and cobinamide kinase) function of two 2; C: cobalamin 5-phosphate synthase; and D: adenosyl transferase. That in order to improve an industrial scale production during fermentation of the precursor thereof, or vitamin B _12, and used to transform Propionibacterium host located on propionic acid bacteria shuttle vector genes encoding enzymes of the four possible.