New assay method for preimplantation factor and preimplantation factor peptide

摘要

Topic Solutions As for this invention, it regards the PIF peptide which is recognized making use of assay method, and its assay method of being used in order to detect the fact that PIF exists. Concretely, as for this invention it regards hurosaitometoriatsusei in order to detect PIF. It is based on the observation that this invention with hurosaitometori which uses the anti- lymphocyte antibody and the anti- blood platelet antibody which it is, the fluorescent sign are done at least showed the increase of rosette formation under the existing of PIF. Furthermore, it is based on also the observation that this invention the monoclonal antibody which under the existing of PIF is connected to CD2 decreasing was shown by hurosaitometori. Furthermore, this invention is regarding PIF peptide, when PIF peptide is added to jar cutting cell culture solution, as for PIF peptide thing, rising the revelation of CD2 in the (ii) jar cutting cell, or makes the liveness of the (iii) jar cutting cell decrease making the connection of the anti- CD2 antibody to the (i) jar cutting cell decrease, it is observed that it is in each case. Furthermore with other example, as for this invention, the case where it connects to the CD2 substrate of the anti- CD2 antibody the ELISA assay which detects PIF by deciding the influence of the test sample, is stipulated.