首页> 外国专利> FLUID CELL AND GENE SEQUENCING REACTION PLATFORM AND GENE SEQUENCING SYSTEM

FLUID CELL AND GENE SEQUENCING REACTION PLATFORM AND GENE SEQUENCING SYSTEM

机译:流体细胞和基因测序反应平台及基因测序系统

摘要

In the invention, a fluid cell, used for sequencing reaction between DNA fragments and reagents, comprises: a reaction chamber, one inner side of which is fixed with multiple DNA fragments; a reagent inlet and a reagent outlet, which are separately located at each end of the other inner side of the reaction chamber and used for reagents flowing into and out from the reaction chamber. Multiple DNA fragments are fixed on the reaction chamber with the small capacity as short tag arrays in sequence and make the reaction go on at the condition of the reagents without diffusion barriers, which improves the contacts of reagents and DNA fragments so as to shorten the reaction time. At the same time, the demands to the concentration and dosage of reagents are low, which reduces the consumption of reagents. As the result, the sequencing cost cut down. Multiple DNA fragments are fixed on the fluid cell at the same time, which provides a way for parallel reaction of various fragments. The automatic high-through sequencing and fast sequencing reactions are achieved in the invention.
机译:在本发明中,一种用于对DNA片段与试剂之间的反应进行测序的流体池,包括:反应室,其内侧固定有多个DNA片段。试剂入口和试剂出口分别位于反应室另一内侧的两端,用于试剂流入和流出反应室。多个DNA片段以短标签序列的顺序固定在反应室中,容量较小,使反应在没有扩散障碍的试剂条件下进行,从而改善了试剂与DNA片段的接触,缩短了反应时间时间。同时,对试剂浓度和剂量的要求较低,这减少了试剂的消耗。结果,降低了测序成本。多个DNA片段同时固定在流体细胞上,这为各种片段的平行反应提供了一种方法。在本发明中实现了自动高通量测序和快速测序反应。

著录项

  • 公开/公告号US2011124094A1

    专利类型

  • 公开/公告日2011-05-26

    原文格式PDF

  • 申请/专利权人 SITONG SHENG;

    申请/专利号US200913054249

  • 发明设计人 SITONG SHENG;

    申请日2009-07-16

  • 分类号C12M1/40;

  • 国家 US

  • 入库时间 2022-08-21 18:14:44

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