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Fusion Tag Comprising an Affinity Tag and an EF-Hand Motif Containing Polypeptide and Methods of Use Thereof
Fusion Tag Comprising an Affinity Tag and an EF-Hand Motif Containing Polypeptide and Methods of Use Thereof
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机译:包含亲和标签和包含多肽的EF-手基序的融合标签及其使用方法
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摘要
The application discloses a fusion tag comprising an affinity tag and a polypeptide comprising one or more EF hand motif(s). Preferably, said fusion tag comprises a polyhistidine tag, one or more EF hand motif(s) of calmodulin and a thrombin cleavage site. Methods of using said fusion tag to purify a polypeptide of interest are also disclosed.
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