Methods for Determining Virulence and Invasiveness Among Various Staphylococcus Aureus Strains

摘要

This invention describes quick diagnostic methods to distinguish CA-MRSA MW2 or CA-MRSA USA300 strains in clinical samples from various sources. Fluorescent labeled primers which amplify 1) two unique agr sequences from each strain, 2) a partial spa gene and 3) a partial pvl gene are packaged into a multiplex PCR kit. A real-time instrumentation to amplify and quantify the PCR amplification products, measured by the change of each of the labeled fluorescent reporter and their thermal melting curve, provides a simple and rapid identification of the most virulent and invasive MRSA strains in the United States.