RECOMBINANT PLASMID DNA PMM 9 ENCODING SYNTHESIS OF PROTEIN A OF STAPHYLOCOCCUS AUREUS, METHOD OF ITS CONSTRUCTION AND THE STRAIN OF BACTERIUM BACILLUS SUBTILIS - A PRODUCER OF PROTEIN A OF STAPHYLOCOCCUS AUREUS

摘要

FIELD: biotechnology, genetic engineering. SUBSTANCE: method involves the use of plasmid pMM 9 carrying protein A gene. This plasmid is stable in different gram-positive microorganisms. Method involves preparing this plasmid also and cloning protein A gene as an intermediate stage in E. coli cells, and also the strain B. subtilis IPM-12 producing protein A into extracellular medium at concentration 100 mg/l cultural fluid, not less. EFFECT: increased level of protein A synthesis of St aureus. 4 cl