Methods to evaluate cytochrome P450 2C19 isoenzyme activity using a breath test

摘要

The present invention relates, generally to a method of determining and assessing cytochrome P450 2C19-related (CYP2Cl9) metabolic capacity in an individual mammalian subject via a breath assay, by determining the relative amount of ¹³CO₂ exhaled by the subject upon intravenous or oral administration of a ¹³C-labeled CYP2C19 substrate compound. The present invention is useful as a non-invasive, in vivo assay for evaluating CYP2C19 enzyme activity in a subject using the metabolite ¹³CO₂ in expired breath, to phenotype individual subjects and to determine the selection, optimal dosage and timing of drug administration.