Method for the detection of Fusarium graminearum

摘要

A method for the detection of Fusarium graminearum (Gibberella zeae) comprising the steps: providing a sample containing a nucleic acid, contacting said sample with at least one forward primer and at least one reverse primer, wherein the at least one reverse primer hybridises within the β-tubulin nucleic acid sequence of Fusarium graminearum (Gibberella zeae) and comprises the nucleic acid sequence 5′-R₁TTTTCGTX₁GX2AGT-3′ (SEQ ID NO 1), wherein R₁ comprises at least one nucleic acid residue of the β-tubulin nucleic acid sequence located upstream of the hybridisation site of the nucleic acid sequence SEQ ID NO 1 and subsequent to the nucleic acid sequence SEQ ID NO 1, X₁ is guanine, adenine or inosine, X₂ is cytosine, adenine or inosine, and wherein the at least one forward primer hybridises upstream of the hybridisation site of a nucleic acid sequence complementary to the at least one reverse primer, subjecting the sample contacted with the at least one forward primer and the at least one reverse primer to a nucleic acid amplification technique, and optionally determining the presence of Fusarium graminearum (Gibberella zeae) in a sample by detecting a nucleic acid amplification product.