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Production of ISOPRENE using Mevalonate (MVA) Pathway and the non Mevalonate (DXP)

机译:使用甲羟戊酸(MVA)途径和非甲羟戊酸(DXP)生产ISOPRENE

摘要

Provides methods for producing ISOPRENE from cultured cells using various components of the DxP Pathway and MVA, or components associated with the path and Route 25 MVA, redox polypeptide that interacts with the accumulation of Iron and sulphur and ISOPRENE synthase. Also provides compositions that include these cultured cells.Methods for production of ISOPRENE are used in the Roofing Industry and rubber.Claim 1: Cells comprising (i) a heterologous Nucleic Acid encoding a polypeptide that interacts with the redox of Iron Sulfur Cluster and a polypeptide of the DxP Pathway, a polypeptide of fa by MVA and a polypeptide of ISOPRENE synthase or (ii) a Duplicate copy of a u00e1cid Nucleic or endogenous redox encodes a polypeptide that interacts with the accumulation of Iron and sulfurA polypeptide of the DxP Pathway, a polypeptide of MVA Pathway and a polypeptide of ISOPRENE synthase.
机译:提供了使用DxP途径和MVA的各种组分或与途径和途径25 MVA相关的组分(与铁和硫和ISOPRENE合酶相互作用的氧化还原多肽)从培养的细胞生产ISOPRENE的方法。还提供了包括这些培养的​​细胞的组合物。ISOPRENE的生产方法用于屋顶工业和橡胶。权利要求1:包含(i)异源核酸的细胞,所述异源核酸编码与铁硫簇的氧化还原相互作用的多肽和多肽DxP途径,MVA的fa多肽和ISOPRENE合酶的多肽,或(ii)核酸或内源氧化还原的重复副本编码与DxP途径的铁和硫的积累相互作用的多肽, MVA途径的多肽和ISOPRENE合酶的多肽。

著录项

  • 公开/公告号AR077115A1

    专利类型

  • 公开/公告日2011-08-03

    原文格式PDF

  • 申请/专利号AR2010P102132

  • 发明设计人

    申请日2010-06-16

  • 分类号C12N1/21;C12P1/04;

  • 国家 AR

  • 入库时间 2022-08-21 18:07:25

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