Isolation of the T-complex distorters and applications thereof for producing transgenic non human animals which a non-Mendelian transmission ratio of a genetic trait

摘要

Disclosed is a method for producing a transgenic non-human male animal , preferably a mammal, fish , bird or insect, wherein the transgene confers a change in the transmission ratio of a genetic trait to the offspring of said non-human male animal, preferably mammal, fish , bird or insect to a non-Mendelian ratio, said method comprising introducing (a) a first nucleic acid molecule encoding the murine t-complex responder Tcr into a chromosome of a non-human germ cell, fertilized egg cell, embryonic cell or a cell derived therefrom, of the same species as the transgenic male to be prepared, said chromosome containing or conferring said genetic trait, thereby linking on said chromosome said murine t-complex responder Tcr to the genetic trait; and (b) at least one second nucleic acid molecule encoding a regulator of the Rho pathway with a Distorter function into a chromosome of a nonhuman germ cell, fertilized egg cell, embryonic cell or a cell derived of the same species as the transgenic male to be prepared, wherein said regulator of the Rho pathway with a Distorter function is a factor involved in G protein signaling, wherein said first nucleic acid molecule encoding the murine t-complex responder Tcr and said at least one second nucleic acid molecule encoding a regulator of the Rho pathway with a Distorter function are introduced into the same or different chromosomes. Further disclosed are non human male or female mammals, fish, birds or insects which are transgenic for the nucleic acid molecules encoding expression product with a Responder and Distorter function and methods of detection of said nucleic acids.