Method for identifying a ligand capable of selectively modulating a functional cascade involving a target, and applications for high throughput screening of molecules of interest.

摘要

Method for identifying a ligand capable of selectively modulating a functional cascade involving a target from a cell of a human, animal or plant organism or a microorganism, involving said functional cascade a succession of reactions in said cell, each one of the dependent reactions of the above and leading to a measurable biological activity in said cell, comprising at least the following steps: a) identifying an intracellular antibody or fragment intracellular antibody, which can be expressed in said cell and comprising at least one of the variable domains of an immunoglobulin chain, capable of binding to said target and modulating said functional cascade involving said target, b) screening from a library of molecules to lower molecular mass