METHODS AND APPARATUS FOR MASS SPECTROMETRY WITH HIGH SAMPLE UTILIZATION

摘要

A method of measuring a mass spectrum with high sample utilization includes mass filtering a first group of precursor ions from a mass spectrum that has a first predetermined range of mass-to-charge ratios. At least one type of precursor ion in the first group of precursor ions is then selectively fragmented. A first fragment mass spectrum of the fragmented precursor ions in the first group of precursor ions is measured while maintaining other precursor ions in the first predetermined range of mass-to-charge ratios. A second group of precursor ions having a second predetermined range of mass-to-charge ratios is mass filtered from the mass spectrum. At least one type of precursor ion is selectively fragmented in the second group of precursor ions. A second fragment mass spectrum of the fragmented precursor ions in the second group of precursor ions is then measured.