Conditioned medium of autologous or allogenic progenitor cells for angiogenesis treatment

摘要

A therapeutic composition is provided that comprises a cell-free conditionedmedium containing mixed secretion products of isolated angiogenic progenitorcells obtained from bone marrow, peripheral blood, or adipose tissue. The compositionmay additionally contain angiogenesis-promoting proteins obtained by transfectingthe progenitors cells in culture with an angiogenesis promoting transgene.The composition is useful to promote angiogenesis when introduced into or adjacentto an ischemic site in a patient, such as in myocardium or peripheral limb. Methodsare also provided for utilizing such cell-free conditioned medium to deliverangiogenesis-promoting proteins to a patient. The cell-free conditioned mediumcan also be injected into the blood stream for delivery to the ischemic tissue.The cells can derive from either an autologous or allogenic source and can be lyophilizedor frozen for storage.