Improved methods for the production of stably transformed, fertile zea mays plants

摘要

The present invention relates to improved methods for the subsequent transformation of at least two DNA constructs into the genome of a Zea mays plant by means of Agrobacterium -mediated transformation, comprising the steps of a first transformation with a first construct comprising a mutated ahas selection marker gene, conferring resistance to imazethapyr but sensitive to imazaquin, and selecting plants resistant to imazethapyr, and a second transformation with a second construct comprising a second mutated ahas selection marker gene conferring resistance to both imazethapyr and imazaquin, and selecting plants resistant to imazaquin.