Mass labels for biomolecules containing a 2,6-dimethyl-piperidin-1-yl methylene or a pyrimidin-2-yl thiomethylene mass marker moiety and a succinimid-oxy-carbonyl reactive functional group

摘要

A mass label for labelling and detecting a biological molecule by mass spectroscopy,which label comprises the following structure: X-L-M wherein X is a mass markermoiety comprising the following group: formula (I), wherein the cyclic unitis aromatic or aliphatic and comprises from 0-3 double bonds independently betweenany two adjacent atoms; each Z is independently N, N(R), C(R),CO, CO(R), C(R), O or S; X is N, C or C(R);each R is independently H, a substituted or unsubstituted straightor branched C-C alkyl group, a substituted or unsubstitutedaliphatic cyclic group, a substituted or unsubstituted aromatic group or a substitutedor unsubstituted heterocyclic group; and y is an integer from 0-10, L is a cleavablelinker comprising an amide bond and M is a mass normalization moiety.