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A METHOD TO DIFFERENTIATE GELATIN CAPSULE OF THE PORCINE SOURCE FROM THE BOVINE SOURCE

机译:从牛源中区分猪源明胶胶囊的方法

摘要

A method to differentiate a gelatin capsule of a porcine source from a bovine source which comprising the steps of: (a) extracting a protein from the gelatin capsule using ammonium sulphate precipitation, wherein the amount of sample used is between 150mg to 400mg, preferably 150mg, which is to be dissolved in a 9mL to HmL, preferably 1OmL, of a liquid such as distilled water at a temperature of between 35°C to 50°C, preferably 40°C; and, (b) separating the protein using gel electrophoresis technique, 7.5% SDS- PAGE, which runs at 200V.
机译:一种从牛源中区分出猪源明胶胶囊的方法,该方法包括以下步骤:(a)使用硫酸铵沉淀从明胶胶囊中提取蛋白质,其中所用样品的量为150mg至400mg,优选为150mg在35℃至50℃,优选40℃的温度下将其溶于9mL至HmL,优选10mL的液体如蒸馏水中; (b)使用凝胶电泳技术分离蛋白质,该蛋白质在200V下运行的7.5%SDS-PAGE。

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