OLIGONUCLEOTIDE APTAMERS TARGETING AT MYCOBACTERIUM TUBERCULOSIS AG85B, PREPARING METHOD AND USES THEREOF

摘要

Oligonucleotide aptamers targeting at Mycobacterium tuberculosis Ag85B, preparing method and uses thereof are disclosed, wherein the oligonucleotide aptamers have one of nucleotide sequences shown in AP-1 to AP-13. The method for preparing the oligonucleotide aptamers comprises: (1) constructing the random single strand DNA (ssDNA) library and primers; (2) preparing random ssDNA library via PCR amplification; (3) SELEX screening; (4) detecting affinity; (5) cloning DNA and sequencing. The oligonucleotide aptamers targeting at Mycobacterium tuberculosis Ag85B are useful for detecting the antigen level of Mycobacterium tuberculosis Ag85B in the human serum, and can be used as substitutes for antibodies to improve the detecting sensitivity and specificity for the antigens of Mycobacterium tuberculosis in the body fluid samples (comprising serum, pleural effusion, cerebrospinal fluid, ascites, and the like), and therefore to improve the accuracy in the diagnosis of tuberculosis.