TRANSFORMATION OF DICOTYLEDONOUS PLANTS BY AN ISOLATED SHOOT APEX MERISTEM METHOD

摘要

Method of transforming dicotyledonous plants, the method comprising: (a) isolating a shoot apex from a seedling; (b) dividing the shoot apex into at least two pieces such that the apical meristem is exposed; (c) making an incision into a meristematic region of the apical meristem; (d) exposing the incised meristematic region to a transformation vector and regenerating a plant from the incised shoot apex.