COMPOSITION FOR ATP SYNTHESIS, MOUNTED FOR GLOEOBACTER RHODOPSIN AND ATP SYNTHASE TO HAVE ORIENTATION

摘要

Disclosed herein is a composition for ATP synthesis, mounted for Gloeobacter rhodopsin (hereinafter, referred to as GR) and ATP synthase to have orientation. Specifically, disclosed herein is a method for synthesizing ATP, including 1) overexpressing GR to translocate protons (H+) from the inside to the outside of the membrane in E. coli, 2) grinding the cells to prepare an inverted membrane, 3) adding Pi and irradiating to form a proton membrane gradient by GR, and 4) synthesizing ATP through ATP synthase, a membrane protein of E. coli from the membrane gradient. The composition for ATP synthesis and the method for preparing ATP according to the present invention may stably provide a large amount of ATP for mass production of biomaterials, and the costs may be greatly reduced due to renewable use of the composition for ATP synthesis according to the present invention.