PROCESS FOR TRYPSIN AND CHYMOTRYPSIN PURIFICATION UTILIZING HYDROPHOBIC INTERACTION CHROMATOGRAPHY

摘要

A method for the separation and purification of trypsin and chymotrypsin in which a crude mixture of trypsin and chymotrypsin is passed through a hydrophobic interaction chromatography media in a chromatographic column. The hydrophobic interaction chromatography media is preferably one having weak anion exchange functionality and alkyl, alkanoyl, phenyl, benzoyl, phenylalkyl or phenylalkanoyl functional groups, such that chymotrypsin is bound to the chromatographic hydrophobic interaction chromatography media and trypsin passes through the media without binding to said media to produce purified trypsin, and thereafter bound chymotrypsin is eluted from the media to produce purified chymotrypsin.