首页> 外国专利> Methods for obtaining thermostable enzymes, DNA polymerase I variants from thermus aquaticus having new catalytic activities, methods for obtaining the same, and applications of the same

Methods for obtaining thermostable enzymes, DNA polymerase I variants from thermus aquaticus having new catalytic activities, methods for obtaining the same, and applications of the same

机译：从具有新催化活性的水生栖热菌中获得热稳定酶，DNA聚合酶I变体的方法，获得该酶的方法及其应用

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摘要

The present invention provides a method for obtaining thermostable enzymes. The present invention also provides variants of DNA polymerase I from Thermus aquaticus. The present invention further provides methods of identifying mutant DNA polymerases having enhanced catalytic activity. The present invention also provides polynucleotides, expression systems, and host cells encoding the mutant DNA polymerases. Still further, the present invention provides a method to carry out reverse transcriptase-polymerase chain reaction (RT-PCR) and kits to facilitate the same.

机译：本发明提供了获得热稳定酶的方法。本发明还提供了水生栖热菌（Thermus aquaticus）的DNA聚合酶I的变体。本发明还提供了鉴定具有增强的催化活性的突变DNA聚合酶的方法。本发明还提供了编码突变DNA聚合酶的多核苷酸，表达系统和宿主细胞。更进一步，本发明提供了进行逆转录酶-聚合酶链反应（RT-PCR）的方法和促进其的试剂盒。

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公开/公告号EP2330119A3

专利类型
公开/公告日2011-09-14

原文格式PDF
申请/专利权人 INSTITUT PASTEUR;CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS);
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申请/专利号EP20100184641
发明设计人 JESTIN JEAN-LUC;VICHIER-GUERRE SOPHIE;FERRIS STÉPHANE;
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申请日2005-02-25
分类号C07K14/435;C12N9/12;
国家 EP
入库时间 2022-08-21 17:54:16

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