DNA EXTRACTION METHOD FOR BURSAPHELENCHUS XYLOPHILUS FROM WOOD CHIPS, LAMP PRIMER SET FOR BURSAPHELENCHUS XYLOPHILUS, AND DETECTION METHOD FOR BURSAPHELENCHUS XYLOPHILUS FROM WOOD CHIPS

摘要

The present invention pieces of wood obtained from plants of Bursaphelenchus xylophilus Pine trees , etc. without separation from wood carving extracting DNA from Bursaphelenchus xylophilus , and to provide a method for detecting the Bursaphelenchus xylophilus . DNA extraction method of the Bursaphelenchus xylophilus of the piece of wood from the collection that contains this Bursaphelenchus xylophilus , LAMP primer set including a primer that anneals to the specific region of DNA, and Bursaphelenchus xylophilus tree that DNA is amplified and detected by the LAMP method using the primer relates to the detection method of Bursaphelenchus xylophilus from pieces .