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ENANTIO-SELETIVE AMIDASE WHICH IS SPECIFIC TO L-TYPE AMINO ACID AMIDE AND DNA ENCODING THE SAME

机译:对映选择性酰胺酶,特异于L型氨基酸酰胺和编码相同氨基酸的DNA

摘要

PURPOSE: A novel enantio-selective amidase which is specific to L-type amino acid amide and a gene encoding the same are provided to ensure remarkably high activity and thermal resistance.;CONSTITUTION: An enantio-selective amidase which enatio-selectively hydrolyses amide bond of L-type amino acid amide contains an amino acid sequence of sequence number 6, or an amino acid sequence in which one or plural amino acid(s) is/are deleted, substituted, or added in the amino acid sequence of sequence number 6. An enantio-selective amidase DNA contains a base sequence encoding a protein with an enzyme activity of enantio-selective amidase. A vector in which the DNA is introduced is pFRPT of deposit number KCCM-10320. A recombinant microorganism transformed by the recombinant vector is deposited by number KCCM11021P.;COPYRIGHT KIPO 2011
机译:目的:提供一种对L型氨基酸酰胺具有特异性的新型对映选择性酰胺酶和编码该酶的基因,以确保显着高的活性和耐热性。;组成:对映选择性水解酰胺键的对映选择性酰胺酶的L-型氨基酸酰胺含有一个序列号6的氨基酸序列,或在序列号6的氨基酸序列中缺失,取代或添加一个或多个氨基酸的氨基酸序列。对映选择性酰胺酶DNA包含编码具有对映选择性酰胺酶的酶活性的蛋白质的碱基序列。引入DNA的载体是保藏号为KCCM-10320的pFRPT。经重组载体转化的重组微生物保藏号为KCCM11021P。; COPYRIGHT KIPO 2011

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