CANDIDA ANTARTICA-DERIVED NOAL LIPASE VARIANT WITH ENHANCED ENZYME ACTIVITY

摘要

PURPOSE: A Candida Antarctica-derived lipase B(CalB) protein variant is provided to increase enzyme activity and to be used in the industry. ;CONSTITUTION: A CalB protein variant is selected from a protein of amino acid sequences 2-8 in which 274-289th amino acid is partially substituted in Candida Antarctica-derived lipase B(CalB) protein. A gene encoding CalB protein variant sequence is denoted by sequence number selected from sequence numbers 10-16. A recombinant vector containing the gene is prepared by inserting the gen into pK7 vector. A transformed cell which produces CalB protein variant is obtained by transforming a host cell with the recombinant vector. The host cells are Pichia pastoris.;COPYRIGHT KIPO 2011