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PREPARATION METHOD OF IN VITRO TRANSGENIC EMBRYO USING MAGNETIC NANOPARTICLE VECTOR SYSTEM COMBINED TO SPERM-MEDIATED GENE TRANSFER METHOD
PREPARATION METHOD OF IN VITRO TRANSGENIC EMBRYO USING MAGNETIC NANOPARTICLE VECTOR SYSTEM COMBINED TO SPERM-MEDIATED GENE TRANSFER METHOD
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机译:磁性纳米粒子矢量系统与精子介导的基因转移法结合制备体外转基因胚胎的方法
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摘要
PURPOSE: A method for preparing in vitro transformed embryos using a nano magnetic sperm system is provided to enhance efficiency of SMGT(sperm-mediated gene transfer) method. CONSTITUTION: A method for preparing in vitro transformed embryos using a nano magnetic sperm system comprises a step of culturing sperms in a conjugate solution containing a conjugate prepared by mixing a foreign gene and nano magnet; a step of maintaining a magnetic field; and a step of applying magnetofection to SMGT method. The nano magnet is ferric oxide(Fe_2O_3, Fe_3O_4), FePt alloy, covalt(Co) or gadolinium(Gd) nanoparticles.
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