Hyaluronic acid immobilized gold nanoparticles for detection of reactive oxygen species and preparing method thereof

摘要

PURPOSE: A method for preparing hyaluronic acid gold nanoparticel sensing oxygen free radical is provided to sensitively react to intracellular free radical and accurately measure the amount of free radical. CONSTITUTION: A method for preparing hyaluronic acid gold nanoparticle sensing oxygen free radical comprises: a step of adding hyaluronidase to hyaluronic acid to obtain low molecular hyaluronic acid; a step of introducing flurescence dye to carboxylic group of the low molecular hyaluronic acid; a step of preparing low molecular hyaluronic acid synthesized material; and a step of fixing the low molecular hyaluronic acid synthesized material on the surface of golf nanoparticle.