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METHOD FOR GENETIC TRANSFORMATION OF HAEMATOCOCCUS PLUVIALIS, STRAIN AND PROTEIN FORMED USING THE SAME

机译:用相同的方法对小球藻进行遗传转化的方法及菌株和蛋白

摘要

The present invention provides transgenic methods of hippocampus Toko Syracuse Alice Ruby platform, which is formed by using transgenic strains and relates to a protein. Transfection method of the hippocampus Toko kusu flat ruby Alice of the present invention, (S1) forming a flat hippocampus Toko kusu ruby Alice culture; (S2) forming an Agrobacterium culture solution; And (S3) the hippocampus Toko kusu flat Ruby Alice Agrobacterium culture medium and characterized in that it comprises the step of mixing and culturing the tumefaciens culture. ; According to the present invention, and expensive equipment or without pre-treatment can be transformed microalgae without material, the foreign gene is introduced directly into the DNA of the chromosome can be maintained stably transformed and can continue to express the gene. Transfection method of microalgae according to the present invention can be produced by converting the various strains of different transformed microalgae, including hippocampus Toko Syracuse, various useful protein isolates prepared as described above and through the bioactive protein which is synthesized by the The mass production of the materials is expected.
机译:本发明提供了通过使用转基因菌株形成的海马Toko Syracuse Alice Ruby平台的转基因方法,涉及一种蛋白质。 (S1)形成扁平海马Toko kusu红宝石爱丽丝培养物; (S2)形成土壤杆菌培养液; (S3)海马Toko kusu扁平红宝石爱丽丝土壤杆菌培养基,其特征在于它包括混合和培养根癌的培养物的步骤。 ;根据本发明,可以在没有材料的情况下用昂贵的设备或无需预处理就可以转化微藻,将外源基因直接引入染色体的DNA中可以保持稳定的转化并可以继续表达该基因。本发明的微藻的转染方法可以通过将不同转化的微藻的各种菌株,包括海马Toko Syracuse,如上所述制备的各种有用的蛋白质分离物,以及通过生物活性蛋白质的合成来生产。材料是预期的。

著录项

  • 公开/公告号KR101034822B1

    专利类型

  • 公开/公告日2011-05-16

    原文格式PDF

  • 申请/专利权人

    申请/专利号KR20080037688

  • 发明设计人 한민호;심상준;

    申请日2008-04-23

  • 分类号C12N15/09;

  • 国家 KR

  • 入库时间 2022-08-21 17:50:16

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