HIV-1 latent cell lines, methods for their preparation and diagnostic methods for latent HIV infection

摘要

PURPOSE: A latent HIV infectious cell line and method for diagnosing HIV infection are provided to show various infection state and study latency and reactivation of HIV provirus. CONSTITUTION: A latent HIV-infectious cell line NCHA-3(deposit number: KCLRF-BP-00185) is prepared by fusing an HIV gene to p14.2 of chromosome 7 of a human T-lymphocyte A3.01 cell line. The HIV gene is pNL4-3 virus gene. The T-lymphocyte is A3.01 cell line. A method for diagnosing latent HIV infection comprises a step of treating PMA(phorbol myristyl acetate) to a latent HIV-infectious cell line to reactivate HIV-provirus, and a step of measuring production amount of HIV-1 p24 antigen. The other method comprises: a step of treaing TNF-a(tumor necrosis factor alpha) or TSA(trichostatin A) to a latent HIV-infectious cell line and a step of measuring acetylation of histone H3 protein.