The present invention CD45 + cells or CD45 + The use of cell culture medium for promoting the proliferation of mesenchymal stem cells, CD45 + cells or CD45 + cells is a composition for promoting growth of mesenchymal stem cells, including CD45 + cells or CD45 + culture medium and cell culture medium to a method of promoting the proliferation of mesenchymal stem cells which comprises culturing the mesenchymal stem cells was added. When mesenchymal stem cells CD45 + CD45 + cells or mesenchymal cells in culture broth was added to the colony-forming ability of mesenchymal stem cells than in the case only a single stem cell culture for more than three times higher. In addition, CD45 + cells or CD45 + cells by treating the culture solution cultured CD29 + cell maintains a unique characteristic, such as mesenchymal stem cells, morphological features, cell markers, as is multipotential. Thus CD45 + cells or CD45 + cell cultures may be used for the growth promotion of mesenchymal stem cells, CD45 + cells or CD45 + cell culture medium by using a method of culture is added to the hMSCs sufficient to use mesenchymal stem cells for cell therapy it is possible to multiply the number.
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